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Vectorstain Elite Abc Hrp Vector Laboratories Pk Pk 6101 Rrids, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Techne corporation recombinant mouse r-spondin 1 protein, cf
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Bio-Rad ahp499g
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Bethyl a303 673a
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Jackson Immuno alexa fluor 647 conjugated jackson immunoreseach
Time-lapse imaging in axonal segments of neurons expressing LAMP1-YFP co-labeled with LysoTracker (LT)-Red (A–C) or expressing LAMP1-mCherry co-labeled with dextran-Oregon Green 488 (OG488) and dextran-Alexa <t>Fluor</t> <t>647</t> <t>(AF647)</t> (D–F).
Alexa Fluor 647 Conjugated Jackson Immunoreseach, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Jackson Immuno anti rabbit biotinylated secondary antibody
Time-lapse imaging in axonal segments of neurons expressing LAMP1-YFP co-labeled with LysoTracker (LT)-Red (A–C) or expressing LAMP1-mCherry co-labeled with dextran-Oregon Green 488 (OG488) and dextran-Alexa <t>Fluor</t> <t>647</t> <t>(AF647)</t> (D–F).
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Image Search Results


KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Post-Golgi carriers, not lysosomes, confer lysosomal properties to pre-degradative organelles in normal and dystrophic axons

doi: 10.1016/j.celrep.2021.109034

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-human CTSD This study N/A Sheep anti-human CTSD Cataldo et al., 1990 N/A Rabbit anti-mouse CTSD Cataldo et al., 2004 N/A Sheep anti-TGN38 Bio-rad Cat# AHP499G; RRID: AB_2203272 Rabbit anti-CTSD Scripps Laboratories Cat# RC245 Goat anti-CTSB Neuromics Cat# GT15047; RRID: AB_2737184 Rabbit anti-CTSL Athens Research & Technology Cat# 01–12-030112; RRID: AB_11190245 Mouse anti-alpha-Tubulin Sigma Cat# T9026; RRID: AB_477593 Rabbit anti-mTOR Cell Signaling Cat# 2983s; RRID: AB_2105622 Rabbit anti-p-mTOR Cell Signaling Cat# 2974s; RRID: AB_2262884 Rabbit anti-p-ATG14 Cell Signaling Cat# 13155; RRID: AB_2798133 Rabbit anti-TFEB Bethyl Laboratories Cat# A303–673A; RRID: AB_11204751 Rabbit anti-TFE3 Sigma Cat# HPA023881; RRID: AB_1857931 Rabbit anti-ubiquitin Dako Cat# Z0458; RRID: AB_2315524 Rabbit anti-LC3 Novus Biologicals Cat# NB100–2220; RRID: AB_10003146 Murine anti-NeuN Millipore Cat# MAB377MI; RRID: AB_2298772 Rabbit anti-GFAP Sigma Cat# G9269; RRID: AB_477035 Rabbit anti-Iba-1 Waco Chemicals Cat# 019–19741; RRID: AB_839504 Rat anti-LAMP1 DSHB Cat# 1D4B; RRID: AB_2134500 Rabbit anti-IGF2R (CI-MPR) Sigma Cat# HPA011332; RRID: AB_1846276 Sheep anti-mouse M6PR (CD-MPR) LSBio Cat# LS- {"type":"entrez-nucleotide","attrs":{"text":"C94323","term_id":"3218938","term_text":"C94323"}} C94323 ; RRID: AB_1935265 Goat anti-mouse IgG, Alexa Fluor 647-conjugated Thermo Fisher Cat# A-21236; RRID: AB_2535805 Goat anti-mouse IgG, Alexa Fluor 488-conjugated Thermo Fisher Cat# A-11029; RRID: AB_138404 Goat anti-rabbit IgG, Alexa Fluor 568-conjugated Thermo Fisher Cat# A-11036; RRID: AB_10563566 Donkey anti-rat IgG, Alexa Fluor 647-conjugated Jackson ImmunoReseach Cat# 712–605-153; RRID: AB_2340694 Donkey anti-rabbit IgG, Alexa Fluor 546-conjugated Thermo Fisher Cat# A-10040; RRID: AB_2534016 Donkey anti-sheep IgG, Alexa Fluor 488-conjugated Thermo Fisher Cat# A-11015; RRID: AB_141362 Goat anti-rabbit IgG, biotinylated Vector Laboratories Cat# BA-1000; RRID: AB_2313606 Goat anti-mouse IgG, biotinylated Vector Laboratories Cat# BA-2000; RRID: AB_2313581 Donkey anti-rabbit IgG, 6 nm gold-conjugated Electron Microscopy Sciences Cat# 25703 Donkey anti-mouse IgG, 6 nm gold-conjugated Electron Microscopy Sciences Cat# 25813 Donkey anti-sheep IgG, 10 nm gold-conjugated Electron Microscopy Sciences Cat# 25825 Chemicals, peptides, and recombinant proteins Sodium cacodylate buffer Electron Microscopy Sciences Cat# 11652 Permount Electron Microscopy Sciences Cat# 17986 Fluoro-Gel Electron Microscopy Sciences Cat# 17985–10 Vectorstain ABC Kit Vector Laboratories Cat# PK-4000 Vector DAB Peroxidase Substrate Vector Laboratories Cat# SK-4100 M.O.M.

Techniques: Plasmid Preparation, Electron Microscopy, Recombinant, Immunodetection, Fluorescence, Transfection, Expressing, Software

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Post-Golgi carriers, not lysosomes, confer lysosomal properties to pre-degradative organelles in normal and dystrophic axons

doi: 10.1016/j.celrep.2021.109034

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-human CTSD This study N/A Sheep anti-human CTSD Cataldo et al., 1990 N/A Rabbit anti-mouse CTSD Cataldo et al., 2004 N/A Sheep anti-TGN38 Bio-rad Cat# AHP499G; RRID: AB_2203272 Rabbit anti-CTSD Scripps Laboratories Cat# RC245 Goat anti-CTSB Neuromics Cat# GT15047; RRID: AB_2737184 Rabbit anti-CTSL Athens Research & Technology Cat# 01–12-030112; RRID: AB_11190245 Mouse anti-alpha-Tubulin Sigma Cat# T9026; RRID: AB_477593 Rabbit anti-mTOR Cell Signaling Cat# 2983s; RRID: AB_2105622 Rabbit anti-p-mTOR Cell Signaling Cat# 2974s; RRID: AB_2262884 Rabbit anti-p-ATG14 Cell Signaling Cat# 13155; RRID: AB_2798133 Rabbit anti-TFEB Bethyl Laboratories Cat# A303–673A; RRID: AB_11204751 Rabbit anti-TFE3 Sigma Cat# HPA023881; RRID: AB_1857931 Rabbit anti-ubiquitin Dako Cat# Z0458; RRID: AB_2315524 Rabbit anti-LC3 Novus Biologicals Cat# NB100–2220; RRID: AB_10003146 Murine anti-NeuN Millipore Cat# MAB377MI; RRID: AB_2298772 Rabbit anti-GFAP Sigma Cat# G9269; RRID: AB_477035 Rabbit anti-Iba-1 Waco Chemicals Cat# 019–19741; RRID: AB_839504 Rat anti-LAMP1 DSHB Cat# 1D4B; RRID: AB_2134500 Rabbit anti-IGF2R (CI-MPR) Sigma Cat# HPA011332; RRID: AB_1846276 Sheep anti-mouse M6PR (CD-MPR) LSBio Cat# LS- {"type":"entrez-nucleotide","attrs":{"text":"C94323","term_id":"3218938","term_text":"C94323"}} C94323 ; RRID: AB_1935265 Goat anti-mouse IgG, Alexa Fluor 647-conjugated Thermo Fisher Cat# A-21236; RRID: AB_2535805 Goat anti-mouse IgG, Alexa Fluor 488-conjugated Thermo Fisher Cat# A-11029; RRID: AB_138404 Goat anti-rabbit IgG, Alexa Fluor 568-conjugated Thermo Fisher Cat# A-11036; RRID: AB_10563566 Donkey anti-rat IgG, Alexa Fluor 647-conjugated Jackson ImmunoReseach Cat# 712–605-153; RRID: AB_2340694 Donkey anti-rabbit IgG, Alexa Fluor 546-conjugated Thermo Fisher Cat# A-10040; RRID: AB_2534016 Donkey anti-sheep IgG, Alexa Fluor 488-conjugated Thermo Fisher Cat# A-11015; RRID: AB_141362 Goat anti-rabbit IgG, biotinylated Vector Laboratories Cat# BA-1000; RRID: AB_2313606 Goat anti-mouse IgG, biotinylated Vector Laboratories Cat# BA-2000; RRID: AB_2313581 Donkey anti-rabbit IgG, 6 nm gold-conjugated Electron Microscopy Sciences Cat# 25703 Donkey anti-mouse IgG, 6 nm gold-conjugated Electron Microscopy Sciences Cat# 25813 Donkey anti-sheep IgG, 10 nm gold-conjugated Electron Microscopy Sciences Cat# 25825 Chemicals, peptides, and recombinant proteins Sodium cacodylate buffer Electron Microscopy Sciences Cat# 11652 Permount Electron Microscopy Sciences Cat# 17986 Fluoro-Gel Electron Microscopy Sciences Cat# 17985–10 Vectorstain ABC Kit Vector Laboratories Cat# PK-4000 Vector DAB Peroxidase Substrate Vector Laboratories Cat# SK-4100 M.O.M.

Techniques: Plasmid Preparation, Electron Microscopy, Recombinant, Immunodetection, Fluorescence, Transfection, Expressing, Software

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: Post-Golgi carriers, not lysosomes, confer lysosomal properties to pre-degradative organelles in normal and dystrophic axons

doi: 10.1016/j.celrep.2021.109034

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-human CTSD This study N/A Sheep anti-human CTSD Cataldo et al., 1990 N/A Rabbit anti-mouse CTSD Cataldo et al., 2004 N/A Sheep anti-TGN38 Bio-rad Cat# AHP499G; RRID: AB_2203272 Rabbit anti-CTSD Scripps Laboratories Cat# RC245 Goat anti-CTSB Neuromics Cat# GT15047; RRID: AB_2737184 Rabbit anti-CTSL Athens Research & Technology Cat# 01–12-030112; RRID: AB_11190245 Mouse anti-alpha-Tubulin Sigma Cat# T9026; RRID: AB_477593 Rabbit anti-mTOR Cell Signaling Cat# 2983s; RRID: AB_2105622 Rabbit anti-p-mTOR Cell Signaling Cat# 2974s; RRID: AB_2262884 Rabbit anti-p-ATG14 Cell Signaling Cat# 13155; RRID: AB_2798133 Rabbit anti-TFEB Bethyl Laboratories Cat# A303–673A; RRID: AB_11204751 Rabbit anti-TFE3 Sigma Cat# HPA023881; RRID: AB_1857931 Rabbit anti-ubiquitin Dako Cat# Z0458; RRID: AB_2315524 Rabbit anti-LC3 Novus Biologicals Cat# NB100–2220; RRID: AB_10003146 Murine anti-NeuN Millipore Cat# MAB377MI; RRID: AB_2298772 Rabbit anti-GFAP Sigma Cat# G9269; RRID: AB_477035 Rabbit anti-Iba-1 Waco Chemicals Cat# 019–19741; RRID: AB_839504 Rat anti-LAMP1 DSHB Cat# 1D4B; RRID: AB_2134500 Rabbit anti-IGF2R (CI-MPR) Sigma Cat# HPA011332; RRID: AB_1846276 Sheep anti-mouse M6PR (CD-MPR) LSBio Cat# LS- {"type":"entrez-nucleotide","attrs":{"text":"C94323","term_id":"3218938","term_text":"C94323"}} C94323 ; RRID: AB_1935265 Goat anti-mouse IgG, Alexa Fluor 647-conjugated Thermo Fisher Cat# A-21236; RRID: AB_2535805 Goat anti-mouse IgG, Alexa Fluor 488-conjugated Thermo Fisher Cat# A-11029; RRID: AB_138404 Goat anti-rabbit IgG, Alexa Fluor 568-conjugated Thermo Fisher Cat# A-11036; RRID: AB_10563566 Donkey anti-rat IgG, Alexa Fluor 647-conjugated Jackson ImmunoReseach Cat# 712–605-153; RRID: AB_2340694 Donkey anti-rabbit IgG, Alexa Fluor 546-conjugated Thermo Fisher Cat# A-10040; RRID: AB_2534016 Donkey anti-sheep IgG, Alexa Fluor 488-conjugated Thermo Fisher Cat# A-11015; RRID: AB_141362 Goat anti-rabbit IgG, biotinylated Vector Laboratories Cat# BA-1000; RRID: AB_2313606 Goat anti-mouse IgG, biotinylated Vector Laboratories Cat# BA-2000; RRID: AB_2313581 Donkey anti-rabbit IgG, 6 nm gold-conjugated Electron Microscopy Sciences Cat# 25703 Donkey anti-mouse IgG, 6 nm gold-conjugated Electron Microscopy Sciences Cat# 25813 Donkey anti-sheep IgG, 10 nm gold-conjugated Electron Microscopy Sciences Cat# 25825 Chemicals, peptides, and recombinant proteins Sodium cacodylate buffer Electron Microscopy Sciences Cat# 11652 Permount Electron Microscopy Sciences Cat# 17986 Fluoro-Gel Electron Microscopy Sciences Cat# 17985–10 Vectorstain ABC Kit Vector Laboratories Cat# PK-4000 Vector DAB Peroxidase Substrate Vector Laboratories Cat# SK-4100 M.O.M.

Techniques: Plasmid Preparation, Electron Microscopy, Recombinant, Immunodetection, Fluorescence, Transfection, Expressing, Software

Time-lapse imaging in axonal segments of neurons expressing LAMP1-YFP co-labeled with LysoTracker (LT)-Red (A–C) or expressing LAMP1-mCherry co-labeled with dextran-Oregon Green 488 (OG488) and dextran-Alexa Fluor 647 (AF647) (D–F).

Journal: Cell reports

Article Title: Post-Golgi carriers, not lysosomes, confer lysosomal properties to pre-degradative organelles in normal and dystrophic axons

doi: 10.1016/j.celrep.2021.109034

Figure Lengend Snippet: Time-lapse imaging in axonal segments of neurons expressing LAMP1-YFP co-labeled with LysoTracker (LT)-Red (A–C) or expressing LAMP1-mCherry co-labeled with dextran-Oregon Green 488 (OG488) and dextran-Alexa Fluor 647 (AF647) (D–F).

Article Snippet: ​ REAGENT OR RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-human CTSD This study N/A Sheep anti-human CTSD Cataldo et al., 1990 N/A Rabbit anti-mouse CTSD Cataldo et al., 2004 N/A Sheep anti-TGN38 Bio-rad Cat# AHP499G; RRID: AB_2203272 Rabbit anti-CTSD Scripps Laboratories Cat# RC245 Goat anti-CTSB Neuromics Cat# GT15047; RRID: AB_2737184 Rabbit anti-CTSL Athens Research & Technology Cat# 01–12-030112; RRID: AB_11190245 Mouse anti-alpha-Tubulin Sigma Cat# T9026; RRID: AB_477593 Rabbit anti-mTOR Cell Signaling Cat# 2983s; RRID: AB_2105622 Rabbit anti-p-mTOR Cell Signaling Cat# 2974s; RRID: AB_2262884 Rabbit anti-p-ATG14 Cell Signaling Cat# 13155; RRID: AB_2798133 Rabbit anti-TFEB Bethyl Laboratories Cat# A303–673A; RRID: AB_11204751 Rabbit anti-TFE3 Sigma Cat# HPA023881; RRID: AB_1857931 Rabbit anti-ubiquitin Dako Cat# Z0458; RRID: AB_2315524 Rabbit anti-LC3 Novus Biologicals Cat# NB100–2220; RRID: AB_10003146 Murine anti-NeuN Millipore Cat# MAB377MI; RRID: AB_2298772 Rabbit anti-GFAP Sigma Cat# G9269; RRID: AB_477035 Rabbit anti-Iba-1 Waco Chemicals Cat# 019–19741; RRID: AB_839504 Rat anti-LAMP1 DSHB Cat# 1D4B; RRID: AB_2134500 Rabbit anti-IGF2R (CI-MPR) Sigma Cat# HPA011332; RRID: AB_1846276 Sheep anti-mouse M6PR (CD-MPR) LSBio Cat# LS- {"type":"entrez-nucleotide","attrs":{"text":"C94323","term_id":"3218938","term_text":"C94323"}} C94323 ; RRID: AB_1935265 Goat anti-mouse IgG, Alexa Fluor 647-conjugated Thermo Fisher Cat# A-21236; RRID: AB_2535805 Goat anti-mouse IgG, Alexa Fluor 488-conjugated Thermo Fisher Cat# A-11029; RRID: AB_138404 Goat anti-rabbit IgG, Alexa Fluor 568-conjugated Thermo Fisher Cat# A-11036; RRID: AB_10563566 Donkey anti-rat IgG, Alexa Fluor 647-conjugated Jackson ImmunoReseach Cat# 712–605-153; RRID: AB_2340694 Donkey anti-rabbit IgG, Alexa Fluor 546-conjugated Thermo Fisher Cat# A-10040; RRID: AB_2534016 Donkey anti-sheep IgG, Alexa Fluor 488-conjugated Thermo Fisher Cat# A-11015; RRID: AB_141362 Goat anti-rabbit IgG, biotinylated Vector Laboratories Cat# BA-1000; RRID: AB_2313606 Goat anti-mouse IgG, biotinylated Vector Laboratories Cat# BA-2000; RRID: AB_2313581 Donkey anti-rabbit IgG, 6 nm gold-conjugated Electron Microscopy Sciences Cat# 25703 Donkey anti-mouse IgG, 6 nm gold-conjugated Electron Microscopy Sciences Cat# 25813 Donkey anti-sheep IgG, 10 nm gold-conjugated Electron Microscopy Sciences Cat# 25825 Chemicals, peptides, and recombinant proteins Sodium cacodylate buffer Electron Microscopy Sciences Cat# 11652 Permount Electron Microscopy Sciences Cat# 17986 Fluoro-Gel Electron Microscopy Sciences Cat# 17985–10 Vectorstain ABC Kit Vector Laboratories Cat# PK-4000 Vector DAB Peroxidase Substrate Vector Laboratories Cat# SK-4100 M.O.M.

Techniques: Imaging, Expressing, Labeling